US 11,676,275 B2
Identifying nucleotides by determining phasing
Francisco Jose Garcia, San Diego, CA (US); Klaus Maisinger, Essex (GB); Stephen Tanner, San Diego, CA (US); John A. Moon, San Diego, CA (US); Tobias Mann, San Diego, CA (US); Michael Lawrence Parkinson, Reyrieux (FR); Anthony James Cox, Essex (GB); and Haifang H. Ge, Essex (GB)
Assigned to Illumina, Inc., San Diego, CA (US)
Filed by Illumina, Inc., San Diego, CA (US)
Filed on Aug. 26, 2021, as Appl. No. 17/445,994.
Application 17/445,994 is a continuation of application No. 17/157,622, filed on Jan. 25, 2021.
Application 17/157,622 is a continuation of application No. 16/378,894, filed on Apr. 9, 2019, abandoned.
Application 16/378,894 is a continuation of application No. 15/354,540, filed on Nov. 17, 2016, granted, now 10,304,189, issued on May 28, 2019.
Application 15/354,540 is a continuation of application No. 14/608,471, filed on Jan. 29, 2015, granted, now 9,530,207, issued on Dec. 27, 2016.
Application 14/608,471 is a continuation of application No. 13/006,206, filed on Jan. 13, 2011, granted, now 8,965,076, issued on Feb. 24, 2015.
Claims priority of provisional application 61/321,029, filed on Apr. 5, 2010.
Claims priority of provisional application 61/294,811, filed on Jan. 13, 2010.
Prior Publication US 2022/0051407 A1, Feb. 17, 2022
Int. Cl. G06T 7/00 (2017.01); G06T 7/33 (2017.01); G06T 7/246 (2017.01); G06T 7/90 (2017.01)
CPC G06T 7/0014 (2013.01) [G06T 7/0012 (2013.01); G06T 7/246 (2017.01); G06T 7/248 (2017.01); G06T 7/33 (2017.01); G06T 7/337 (2017.01); G06T 7/90 (2017.01); G06T 2207/10024 (2013.01); G06T 2207/30024 (2013.01); G06T 2207/30072 (2013.01)] 24 Claims
OG exemplary drawing
 
1. A system for base calling a nucleotide in a nucleic acid molecule, comprising:
a processor;
a storage device; and
a program comprising instructions for:
acquiring fluorescent emissions from labeled nucleotides hybridized to a cluster of identical nucleic acids bound to a substrate;
calculating the fraction of labeled nucleotides in the cluster that are out of phase;
identifying a particular base called nucleotide based on the acquired fluorescent emissions and the calculated fraction of labeled nucleotides that are out of phase; and
assigning a quality score to the particular base called nucleotide based on the identification.