CPC G06T 7/0014 (2013.01) [G06T 7/0012 (2013.01); G06T 7/246 (2017.01); G06T 7/248 (2017.01); G06T 7/33 (2017.01); G06T 7/337 (2017.01); G06T 7/90 (2017.01); G06T 2207/10024 (2013.01); G06T 2207/30024 (2013.01); G06T 2207/30072 (2013.01)] | 24 Claims |
1. A system for base calling a nucleotide in a nucleic acid molecule, comprising:
a processor;
a storage device; and
a program comprising instructions for:
acquiring fluorescent emissions from labeled nucleotides hybridized to a cluster of identical nucleic acids bound to a substrate;
calculating the fraction of labeled nucleotides in the cluster that are out of phase;
identifying a particular base called nucleotide based on the acquired fluorescent emissions and the calculated fraction of labeled nucleotides that are out of phase; and
assigning a quality score to the particular base called nucleotide based on the identification.
|