CPC G01N 33/743 (2013.01) [G01N 30/50 (2013.01); G01N 33/6848 (2013.01)] | 15 Claims |
1. A method for determining the amount of testosterone in each of a plurality of human samples with a single mass spectrometric assay, the method comprising:
i) subjecting each of a plurality of human samples to a different derivatizing agent to generate a differently derivatized testosterone in each of the plurality of samples, wherein the derivatization agents comprise hydroxylamine chloride and methoxylamine chloride;
ii) combining the plurality of samples to form a multiplex sample; and
iii) quantifying the amount of testosterone in each sample by mass spectrometry, wherein the limit of quantitation of the methods is less than or equal to 1 ng/dL.
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