US 11,674,969 B2
Mass spectrometric determination of testosterone in multiplexed patient samples
Mildred M. Goldman, Laguna Niguel, CA (US); Scott Goldman, Laguna Niguel, CA (US); Julia Colletti, Mission Viejo, CA (US); and Nigel Clarke, Vista, CA (US)
Assigned to Quest Diagnostics Investments LLC, Secaucus, NJ (US)
Filed by Quest Diagnostics Investments LLC, Secaucus, NJ (US)
Filed on Mar. 15, 2019, as Appl. No. 16/355,552.
Claims priority of provisional application 62/644,351, filed on Mar. 16, 2018.
Prior Publication US 2019/0285654 A1, Sep. 19, 2019
Int. Cl. G01N 33/74 (2006.01); G01N 33/68 (2006.01); G01N 30/50 (2006.01)
CPC G01N 33/743 (2013.01) [G01N 30/50 (2013.01); G01N 33/6848 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A method for determining the amount of testosterone in each of a plurality of human samples with a single mass spectrometric assay, the method comprising:
i) subjecting each of a plurality of human samples to a different derivatizing agent to generate a differently derivatized testosterone in each of the plurality of samples, wherein the derivatization agents comprise hydroxylamine chloride and methoxylamine chloride;
ii) combining the plurality of samples to form a multiplex sample; and
iii) quantifying the amount of testosterone in each sample by mass spectrometry, wherein the limit of quantitation of the methods is less than or equal to 1 ng/dL.