US 11,674,870 B2
Sample protection method
Bipin Gupta, Pleasanton, CA (US); and Marc Key, Ojai, CA (US)
Assigned to Diagnostic BioSystems, Pleasanton, CA (US); and Marc Key, Ojai, CA (US)
Filed by Bipin Gupta, Pleasanton, CA (US); and Marc Key, Ojai, CA (US)
Filed on Jan. 23, 2020, as Appl. No. 16/750,148.
Claims priority of provisional application 62/888,822, filed on Aug. 19, 2019.
Prior Publication US 2021/0055189 A1, Feb. 25, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 1/30 (2006.01); G01N 1/28 (2006.01); G01N 33/483 (2006.01); G01N 1/44 (2006.01); C08G 65/332 (2006.01); G01N 1/42 (2006.01); C09D 171/02 (2006.01); C09D 129/04 (2006.01); C09D 105/04 (2006.01); G02B 21/34 (2006.01); C08L 71/02 (2006.01); C08L 29/04 (2006.01)
CPC G01N 1/30 (2013.01) [C08G 65/3326 (2013.01); C08L 29/04 (2013.01); C08L 71/02 (2013.01); C09D 105/04 (2013.01); C09D 129/04 (2013.01); C09D 171/02 (2013.01); G01N 1/28 (2013.01); G01N 1/2813 (2013.01); G01N 1/42 (2013.01); G01N 1/44 (2013.01); G01N 33/4833 (2013.01); G01N 2001/302 (2013.01); G02B 21/34 (2013.01)] 10 Claims
OG exemplary drawing
 
1. A sample protection method for reducing detachment of a sectioned sample from a microscope slide during target retrieval performed on the sectioned sample, the method comprising the steps performed in the following sequential order:
applying a protecting reagent onto the sectioned sample that is in contact with the microscope slide, the sectioned sample having been removed from an embedding medium;
drying the protecting reagent; and
performing target retrieval on the sectioned sample,
wherein the protecting reagent is polyethylene glycol or alginic acid,
wherein the target retrieval comprises heat-induced antigen retrieval, and
wherein detachment of the sectioned sample is reduced during heat-induced antigen retrieval.