US 11,674,190 B2
Methods and compositions for identifying mutations that decrease expression of PPO11
Michele Feikert, St. Louis, MO (US); Byron Froman, Davis, CA (US); Graeme S. Garvey, St. Louis, MO (US); Leo Kelly, St. Louis, MO (US); and William Waycott, San Luis Obispo, CA (US)
Assigned to Seminis Vegetable Seeds, Inc., St. Louis, MO (US)
Filed by Seminis Vegetable Seeds, Inc., St. Louis, MO (US)
Filed on May 26, 2020, as Appl. No. 16/883,738.
Application 16/167,275 is a division of application No. 15/343,061, filed on Nov. 3, 2016, granted, now 10,106,859, issued on Oct. 23, 2018.
Application 15/343,061 is a division of application No. 14/035,756, filed on Sep. 24, 2013, granted, now 9,506,076, issued on Nov. 29, 2016.
Application 16/883,738 is a continuation of application No. 16/167,275, filed on Oct. 22, 2018, granted, now 10,689,714.
Claims priority of provisional application 61/704,602, filed on Sep. 24, 2012.
Prior Publication US 2020/0392590 A1, Dec. 17, 2020
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6895 (2018.01); C12Q 1/686 (2018.01); A01N 65/12 (2009.01); C12N 15/82 (2006.01)
CPC C12Q 1/6895 (2013.01) [A01N 65/12 (2013.01); C12N 15/8218 (2013.01); C12Q 1/686 (2013.01)] 8 Claims
 
1. A method for identifying plants having reduced expression or lacking expression of a PPO11 gene, comprising:
a) obtaining a plurality of plants at least one of which comprises a mutation that results in decreased expression of the PPO11 gene relative to other members of the plurality of plants;
b) screening the plurality of plants to identify the mutation that results in said decreased expression of the PPO11 gene;
c) selecting said plant that comprises said mutation,
wherein the PPO11 gene has at least 95% sequence identity to SEQ ID NO:9.