CPC C12Q 1/6886 (2013.01) [C12N 15/11 (2013.01); C12Q 1/6876 (2013.01); C12Q 2600/154 (2013.01); C12Q 2600/156 (2013.01); C12Q 2600/166 (2013.01)] | 7 Claims |
1. A run control composition, comprising:
a) a number of copies of a first synthetic DNA fragment comprising a sequence of a portion of a human biomarker DNA, wherein the sequence is synthesized to comprise a sequence region containing a number and pattern of cytosines and cytosine-phosphate-guanosine (CpG) dinucleotides in which each of the cytosines in the CpG dinucleotides in the pattern comprises a 5-methyl;
b) a number of copies of a second synthetic DNA fragment comprising the same sequence of the same portion of the human biomarker DNA as the first synthetic DNA, and comprising a sequence region comprising the same number and pattern of cytosines and CpG dinucleotides as the sequence region in the first synthetic DNA fragment, wherein none of the cytosines in the pattern comprises a 5-methyl; and
c) fish DNA.
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