US 11,674,174 B2
Nucleic acid sequences using tags
Sergey Kalachikov, New York, NY (US); Jingyue Ju, New York, NY (US); Irina Morozova, New York, NY (US); and Michael Dorwart, Mountain View, CA (US)
Assigned to THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK, New York, NY (US); and ROCHE SEQUENCING SOLUTIONS, INC., Pleasanton, CA (US)
Filed by Sergey Kalachikov, New York, NY (US); Jingyue Ju, New York, NY (US); Irina Morozova, New York, NY (US); and Michael Dorwart, Mountain View, CA (US)
Filed on Jan. 6, 2020, as Appl. No. 16/735,067.
Application 16/735,067 is a division of application No. 15/449,757, filed on Mar. 3, 2017, granted, now 10,526,647.
Application 15/449,757 is a division of application No. 14/073,445, filed on Nov. 6, 2013, granted, now 9,605,309, issued on Mar. 28, 2017.
Claims priority of provisional application 61/880,407, filed on Sep. 20, 2013.
Claims priority of provisional application 61/737,621, filed on Dec. 14, 2012.
Claims priority of provisional application 61/724,869, filed on Nov. 9, 2012.
Prior Publication US 2020/0248250 A1, Aug. 6, 2020
Int. Cl. C12Q 1/6869 (2018.01); G01N 33/487 (2006.01); G01N 27/447 (2006.01)
CPC C12Q 1/6869 (2013.01) [G01N 27/44717 (2013.01); G01N 27/44791 (2013.01); G01N 33/48721 (2013.01)] 10 Claims
 
1. A method for generating a nanopore-polymerase complex, the method comprising:
(a) obtaining a nanopore composition enriched for nanopores having a first subunit and a second subunit in a ratio of 1:n−1, wherein n is the total number of subunits comprising the nanopore;
(b) attaching a polymerase to the first subunit to obtain the nanopore-polymerase complex,
wherein the polymerase is attached to the first subunit by a protein-protein tag.