CPC C12Q 1/6869 (2013.01) [G01N 27/44717 (2013.01); G01N 27/44791 (2013.01); G01N 33/48721 (2013.01)] | 10 Claims |
1. A method for generating a nanopore-polymerase complex, the method comprising:
(a) obtaining a nanopore composition enriched for nanopores having a first subunit and a second subunit in a ratio of 1:n−1, wherein n is the total number of subunits comprising the nanopore;
(b) attaching a polymerase to the first subunit to obtain the nanopore-polymerase complex,
wherein the polymerase is attached to the first subunit by a protein-protein tag.
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