CPC C12Q 1/6806 (2013.01) [B01D 33/15 (2013.01); B01D 33/155 (2013.01); B01L 3/00 (2013.01); B01L 3/5021 (2013.01); B03C 1/30 (2013.01); B04B 3/00 (2013.01); C12N 15/1006 (2013.01); C12N 15/1013 (2013.01); C12N 15/1017 (2013.01); C12Q 1/6886 (2013.01); C12Q 2600/158 (2013.01); C12Q 2600/16 (2013.01); Y10T 436/143333 (2015.01)] | 11 Claims |
1. A method for partitioning a human stool homogenate comprising human DNA and bacterial DNA to produce a human target DNA preparation and a residual supernatant, the method comprising:
a) treating a fluid fraction from a human stool homogenate with insoluble polyvinylpyrrolidone to bind inhibitor, if present, in an insoluble inhibitor complex, to produce a treated stool fluid;
b) exposing the treated stool fluid to a target sequence-specific capture reagent comprising particles attached to oligonucleotides complementary to at least a portion of first human target DNAs under conditions wherein the first target sequence-specific capture reagent forms first capture complexes comprising the target sequence-specific capture reagent and the first human target DNAs;
c) separating the first capture complexes from the treated stool fluid to produce:
i) a human target DNA preparation comprising the first human target DNAs captured in first capture complexes in step b); and
ii) a residual supernatant comprising bacterial DNA and human target DNAs not complementary to the oligonucleotides of the target-sequence specific capture reagent used in step b).
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