CPC C12P 13/001 (2013.01) [C12N 9/78 (2013.01); C12N 9/80 (2013.01); C12N 9/86 (2013.01); C12N 15/52 (2013.01); C12N 15/70 (2013.01); C12N 15/81 (2013.01); C12P 7/6463 (2013.01); C12Y 305/01054 (2013.01); C12Y 305/01084 (2013.01); C12Y 305/02015 (2013.01); C12Y 305/04 (2013.01); C12Y 305/04003 (2013.01); C12Y 305/99004 (2013.01)] | 20 Claims |
1. A composition comprising
a) a fermentation mixture comprising
i) a first fraction consisting essentially of a fraction of a fractionated grain, and
ii) a second fraction comprising one or more nitrogen-containing compounds; and
b) a transformed cell comprising an exogenous nucleic acid molecule that encodes an enzyme for metabolizing the one or more nitrogen-containing compounds as a source of nitrogen; wherein the one or more nitrogen-containing compounds cannot be metabolized by a native, untransformed cell of the same species, and wherein the enzyme is a melamine deaminase (EC 3.5.4.45), a guanine deaminase (EC 3.5.4.3), a N-isopropylammelide isopropylamino (Ammelide) hydrolyase (EC 3.5.99.4), a cyanuric acid hydrolyase (EC 3.5.2.15), a biuret amidohydrolase (EC 3.5.1.84), an allophanate hydrolyase (EC 3.5.1.54), a cyanamide hydratase (EC 4.2.1.69), a urease (EC 3.5.1.5), or a urea carboxylase (EC 6.3.4.6).
|