US 11,674,162 B2
Selective advantage in fermentation
Emily H. Greenhagen, Melrose, MA (US); Maureen Hamilton, Littleton, MA (US); William G. LaTouf, Belmont, MA (US); Andrew L. Consiglio, Holden, MA (US); Kyle M. MacEwen, Braintree, MA (US); and Arthur J. Shaw, IV, Belmont, MA (US)
Assigned to Ginkgo Bioworks, Inc., Boston, MA (US)
Appl. No. 15/303,330
Filed by Ginkgo Bioworks, Inc., Boston, MA (US)
PCT Filed Apr. 8, 2015, PCT No. PCT/US2015/024943
§ 371(c)(1), (2) Date Oct. 11, 2016,
PCT Pub. No. WO2015/157431, PCT Pub. Date Oct. 15, 2015.
Claims priority of provisional application 62/083,540, filed on Nov. 24, 2014.
Claims priority of provisional application 61/976,672, filed on Apr. 8, 2014.
Prior Publication US 2017/0096691 A1, Apr. 6, 2017
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 9/80 (2006.01); C12N 9/88 (2006.01); C12N 9/78 (2006.01); C12P 13/00 (2006.01); C12P 7/6463 (2022.01); C12N 9/86 (2006.01); C12N 15/52 (2006.01); C12N 15/70 (2006.01); C12N 15/81 (2006.01)
CPC C12P 13/001 (2013.01) [C12N 9/78 (2013.01); C12N 9/80 (2013.01); C12N 9/86 (2013.01); C12N 15/52 (2013.01); C12N 15/70 (2013.01); C12N 15/81 (2013.01); C12P 7/6463 (2013.01); C12Y 305/01054 (2013.01); C12Y 305/01084 (2013.01); C12Y 305/02015 (2013.01); C12Y 305/04 (2013.01); C12Y 305/04003 (2013.01); C12Y 305/99004 (2013.01)] 20 Claims
 
1. A composition comprising
a) a fermentation mixture comprising
i) a first fraction consisting essentially of a fraction of a fractionated grain, and
ii) a second fraction comprising one or more nitrogen-containing compounds; and
b) a transformed cell comprising an exogenous nucleic acid molecule that encodes an enzyme for metabolizing the one or more nitrogen-containing compounds as a source of nitrogen; wherein the one or more nitrogen-containing compounds cannot be metabolized by a native, untransformed cell of the same species, and wherein the enzyme is a melamine deaminase (EC 3.5.4.45), a guanine deaminase (EC 3.5.4.3), a N-isopropylammelide isopropylamino (Ammelide) hydrolyase (EC 3.5.99.4), a cyanuric acid hydrolyase (EC 3.5.2.15), a biuret amidohydrolase (EC 3.5.1.84), an allophanate hydrolyase (EC 3.5.1.54), a cyanamide hydratase (EC 4.2.1.69), a urease (EC 3.5.1.5), or a urea carboxylase (EC 6.3.4.6).