CPC C12P 7/22 (2013.01) [C12N 9/0077 (2013.01); C12N 9/0079 (2013.01); C12P 17/06 (2013.01); C12Y 114/15004 (2013.01)] | 11 Claims |
1. A modified P450 monooxygenase having an amino acid sequence with at least 90% sequence identity with SEQ ID NO: 1, wherein the asparagine at position 199 of the amino acid sequence of SEQ ID NO. 1 is substituted by an amino acid selected from the group consisting of glutamine, isoleucine, leucine, phenylalanine, histidine, methionine, arginine, serine, threonine, tyrosine, tryptophan, alanine, valine, and lysine to provide a functional mutation, wherein said functional mutation leads to an improved reactivity on hydroxylation of aromatic hydrocarbons as compared to a non-modified P450 monooxygenase.
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