CPC C12N 7/00 (2013.01) [C12N 1/205 (2021.05); C12N 2795/00021 (2013.01); C12N 2795/00051 (2013.01); C12R 2001/01 (2021.05)] | 29 Claims |
1. A method of producing a mutant phage with expanded host range, comprising:
(i) preparing a plurality of co-culture mixtures of varying ratios, comprising a host bacterial strain and a target-host bacterial strain;
(ii) adding a phage strain to each of the plurality of the co-culture mixtures;
(iii) incubating the plurality of the co-culture mixtures and the phage strain under bacterial culture conditions;
(iv) collecting a phage lysate from each of the plurality of the co-culture mixtures;
(v) pooling the collected phage lysates from each of the plurality of the co-culture mixtures;
(vi) assaying the pooled phage lysates to determine if host phage of the phage strain has expanded; and,
(vii) isolating the mutant phage with expanded host range,
wherein the mutant bacteriophage with expanded host range is a recombinant mutant bacteriophage with expanded host range comprising an indicator gene inserted into a late gene region of genome.
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