	US 11,674,117 B2
	Streptococcus thermophilus starter cultures
Roelof Hendrik Matthijs Kouwen, Echt (NL); Douwe Van Sinderen, D. Cork (IE); Brian McDonnell, Cork (IE); Pieter Emiel Verloren Van Themaat, Echt (NL); and Jennifer Mahony, Echt (NL)
Assigned to DSM IP ASSETS B.V., Heerlen (NL)
Appl. No. 16/470,856
Filed by DSM IP Assets B.V., Heerlen (NL)
PCT Filed Dec. 14, 2017, PCT No. PCT/EP2017/082776 § 371(c)(1), (2) Date Jun. 18, 2019, PCT Pub. No. WO2018/114573, PCT Pub. Date Jun. 28, 2018.
Claims priority of application No. 16205245 (EP), filed on Dec. 20, 2016.
Prior Publication US 2019/0367866 A1, Dec. 5, 2019
Int. Cl. C12N 1/20 (2006.01); A23C 9/123 (2006.01); C12Q 1/689 (2018.01); C12R 1/46 (2006.01); G16B 35/20 (2019.01)

CPC C12N 1/20 (2013.01) [A23C 9/1238 (2013.01); C12N 1/205 (2021.05); C12Q 1/689 (2013.01); G16B 35/20 (2019.02); A23Y 2240/75 (2013.01); C12R 2001/46 (2021.05)]

12 Claims

1. A starter culture comprising at least a first Streptococcus thermophilus strain and a second Streptococcus thermophilus strain, wherein:

the first and second Streptococcus thermophilus strains are chosen from the groups consisting of rhamnose glucose polysaccharide (RGP) group 1, RGP group 2, RGP group 3, and RGP group 4, with the proviso that the first and second Streptococcus thermophilus strains do not belong to the same RGP group;

and wherein:

RGP group 1 is defined as the group of Streptococcus thermophilus strains which allow generation of an amplicon by using a primer pair having nucleotide sequences SEQ ID NO:1 and SEQ ID NO:2 in a PCR reaction;

RGP group 2 is defined as the group of Streptococcus thermophilus strains which allow generation of an amplicon by using a primer pair having nucleotide sequences SEQ ID NO:4 and SEQ ID NO:5 in a PCR reaction;

RGP group 3 is defined as the group of Streptococcus thermophilus strains which allow generation of an amplicon by using a primer pair having nucleotide sequences SEQ ID NO:7 and SEQ ID NO:8 in a PCR reaction; and

RGP group 4 is defined as the group of Streptococcus thermophilus strains which allow to generation of an amplicon by using a primer pair having nucleotide sequences SEQ ID NO:10 and SEQ ID NO:11 in a PCR reaction.