US 11,673,940 B2
Recombinant production of a collagen peptide preparation and use thereof
Stephan Hausmanns, Heidelberg (DE); Hans-Ulrich Frech, Weinheim (DE); Steffen Oesser, Glücksburg (DE); and Martin Hahn, Gronau (DE)
Assigned to Gelita AG, Eberbach (DE)
Appl. No. 17/287,905
Filed by Gelita AG, Eberbach (DE)
PCT Filed Nov. 6, 2019, PCT No. PCT/EP2019/080421
§ 371(c)(1), (2) Date Apr. 22, 2021,
PCT Pub. No. WO2020/094728, PCT Pub. Date May 14, 2020.
Claims priority of application No. 10 2018 218 916.1 (DE), filed on Nov. 6, 2018; application No. 10 2019 200 790.2 (DE), filed on Jan. 23, 2019; and application No. 10 2019 202 606.0 (DE), filed on Feb. 26, 2019.
Prior Publication US 2021/0309723 A1, Oct. 7, 2021
Int. Cl. C07K 14/78 (2006.01); A61K 8/65 (2006.01); A23L 33/18 (2016.01); A61Q 3/00 (2006.01); A61Q 5/00 (2006.01); A61Q 19/00 (2006.01); A61K 38/00 (2006.01)
CPC C07K 14/78 (2013.01) [A23L 33/18 (2016.08); A61K 8/65 (2013.01); A61Q 3/00 (2013.01); A61Q 5/00 (2013.01); A61Q 19/00 (2013.01); A23V 2002/00 (2013.01); A61K 38/00 (2013.01)] 13 Claims
 
1. A method for producing a collagen peptide preparation, comprising:
a) expressing, from a coding nucleotide sequence, a collagen peptide having a molecular weight in a range from 8 to 100 kDa, and
b) enzymatically hydrolyzing the expressed collagen peptide under conditions to produce a collagen peptide preparation comprising hydrolyzed collagen peptides having an average molecular weight of 1 to 5 kDa, wherein
no more than 5.5 wt.-% of the hydrolyzed collagen peptides have a molecular weight less than 500 Da,
no more than 2.8 wt.-% of the hydrolyzed collagen peptides have a molecular weight between 7500 Da and 13500 Da, and
from 35 to 60 wt.-% of the hydrolyzed collagen peptides have a molecular weight between 1500 Da and 3500 Da.