US 11,673,921 B2
Cell-free protein synthesis platform derived from cellular extracts of Vibrio natriegens
Michael Christopher Jewett, Evanston, IL (US); Benjamin James Des Soye, Evanston, IL (US); and Samuel Ryan Davidson, Evanston, IL (US)
Assigned to Northwestern University, Evanston, IL (US)
Appl. No. 16/762,889
Filed by Northwestern University, Evanston, IL (US)
PCT Filed Nov. 12, 2018, PCT No. PCT/US2018/060279
§ 371(c)(1), (2) Date May 8, 2020,
PCT Pub. No. WO2019/094859, PCT Pub. Date May 16, 2019.
Claims priority of provisional application 62/584,406, filed on Nov. 10, 2017.
Prior Publication US 2021/0171584 A1, Jun. 10, 2021
Int. Cl. C07K 14/28 (2006.01); C07K 1/00 (2006.01); C12N 1/20 (2006.01)
CPC C07K 14/28 (2013.01) [C07K 1/00 (2013.01); C12N 1/20 (2013.01)] 21 Claims
 
1. A platform for in vitro transcription of mRNA and/or translation of peptides, polypeptides, or sequence defined polymers, the platform comprising a cellular extract prepared from a cell culture of a species of Vibrio; wherein the Vibrio cells in the culture are in stationary phase immediately prior to extract preparation.