	US 11,673,911 B2
	Stabilized nucleic acids encoding messenger ribonucleic acid (mRNA)
Christian Dombrowski, Auburndale, MA (US)
Assigned to Intellia Therapeutics, Inc., Cambridge, MA (US)
Filed by Intellia Therapeutics, Inc., Cambridge, MA (US)
Filed on Feb. 14, 2020, as Appl. No. 16/791,076.
Application 16/791,076 is a continuation of application No. PCT/US2018/046772, filed on Aug. 14, 2018.
Claims priority of provisional application 62/545,883, filed on Aug. 15, 2017.
Prior Publication US 2020/0172564 A1, Jun. 4, 2020
Int. Cl. C12N 15/11 (2006.01); C07H 21/02 (2006.01); C12N 15/63 (2006.01); C07K 14/475 (2006.01); C07K 14/52 (2006.01); C12N 9/22 (2006.01)

CPC C07H 21/02 (2013.01) [C12N 15/11 (2013.01); C12N 15/63 (2013.01); C07K 14/475 (2013.01); C07K 14/521 (2013.01); C12N 9/22 (2013.01)]

22 Claims

1. A DNA comprising nucleotides encoding a poly-adenylated (poly-A) tail located 3′ to nucleotides encoding a protein of interest, wherein the poly-A tail comprises:

a. three or more homopolymer sequences of at least 8 consecutive adenine (A) nucleotides; and

b. an interrupting sequence comprising:

i. one non-adenine nucleotide; or

ii. a consecutive stretch of 2-10 non-adenine nucleotides, between each homopolymer sequence.