	US 11,672,261 B2
	*Compositions comprising Fabaceae* family plant components, processes of preparation and uses thereof**
Pierre Lavallée, Deschambault-Grondines (CA); Réjean Desgagnés, St-Jean-Chrysostome (CA); Laurence Cambron-Fortin, Québec (CA); Louis-Philippe Vézina, Neuville (CA); and Pierre Talbot, Notre-dame-du-Portage (CA)
Assigned to VIRENTIA INNOVATION INC., Québec (CA)
Appl. No. 17/603,336
Filed by VIRENTIA INNOVATION INC., Québec (CA)
PCT Filed Apr. 16, 2020, PCT No. PCT/CA2020/050507 § 371(c)(1), (2) Date Oct. 12, 2021, PCT Pub. No. WO2020/210907, PCT Pub. Date Oct. 22, 2020.
Claims priority of provisional application 62/835,156, filed on Apr. 17, 2019.
Prior Publication US 2022/0211073 A1, Jul. 7, 2022
Int. Cl. A23J 1/14 (2006.01); A23K 10/30 (2016.01); A23L 33/105 (2016.01)

CPC A23J 1/14 (2013.01) [A23K 10/30 (2016.05); A23L 33/105 (2016.08); A23V 2002/00 (2013.01)]

24 Claims

1. A process for preparing a dry chloroplast composition, the dry chloroplast composition comprising chloroplast from Fabaceae family plants, said process comprising:

providing Fabaceae family plant fragments;

separating a macrofiber depleted suspension from the plant fragments;

separating the plant microfibers from the macrofiber depleted suspension;

obtaining a microfiber depleted suspension;

separating chloroplasts from the microfiber depleted suspension;

obtaining the chloroplast suspension;

washing the chloroplast suspension; and

conditioning the washed chloroplast suspension to obtain the dry chloroplast composition, wherein the conditioning comprises:

mixing the washed chloroplast suspension with a conservation agent and/or antioxidant, and drying the washed chloroplast suspension to obtain the dry chloroplast composition,

mixing the washed chloroplast suspension with a formulating agent, a conservation agent, a food supplement, an omega-3 fatty acid, an omega-6 fatty acid or mixtures thereof, and drying and granulating the washed chloroplast suspension to obtain the dry chloroplast composition,

and wherein the conditioning comprises packaging the dry chloroplast composition,

wherein at least 75% of the chloroplasts comprised in the composition are intact as determined by dynamic light scattering measurement and analysis, wherein the separating of the chloroplasts from the microfiber depleted suspension comprises sedimenting the chloroplasts and optionally isolating the sedimented chloroplasts;

wherein the chloroplasts are sedimented at a pH of about 4.0 to about 5.5:

and

optionally wherein the washed chloroplast suspension and/or the dry chloroplast composition comprise about 90% of the Rubisco content in the chloroplast suspension.