US 11,670,494 B2
Systems and methods for performing tandem mass spectrometry
Philip M. Remes, Livermore, CA (US); and Michael W. Senko, Sunnyvale, CA (US)
Filed by Thermo Finnigan LLC, San Jose, CA (US)
Filed on Dec. 28, 2020, as Appl. No. 17/135,749.
Prior Publication US 2022/0208535 A1, Jun. 30, 2022
Int. Cl. H01J 49/00 (2006.01); G01N 30/72 (2006.01); G01N 30/86 (2006.01); G01N 30/02 (2006.01)
CPC H01J 49/0081 (2013.01) [G01N 30/7233 (2013.01); G01N 30/8631 (2013.01); H01J 49/0036 (2013.01); G01N 2030/027 (2013.01)] 31 Claims
OG exemplary drawing
 
1. A method of performing tandem mass spectrometry comprising:
supplying a sample to a chromatography column;
directing components included in the sample and eluting from the chromatography column to a mass spectrometer;
acquiring a series of mass spectra including intensity values of ions produced from the components as a function of m/z of the ions;
extracting, from the series of mass spectra, a plurality of detection points representing intensity as a function of time for a selected m/z;
estimating, based on the plurality of detection points extracted from the series of mass spectra and using an algorithm trained to estimate a normalized intensity value of selected detection points, a relative position of a selected detection point included in the plurality of detection points, the relative position of the selected detection point representing a position of the selected detection point relative to an expected reference point; and
performing, at the mass spectrometer and based on the estimated relative position of the selected detection point relative to an expected reference point, a dependent acquisition for the selected m/z.