US 11,668,718 B2
Peptide quantitation assay for differentiating full-length high molecular weight kininogen (HMWK) and cleaved HMWK
Jiang Wu, Lexington, MA (US); Guodong Zhang, Lexington, MA (US); Daniel J. Sexton, Melrose, MA (US); Ryan Faucette, Melrose, MA (US); Gul M. Mustafa, Morgantown, WV (US); and Mark Szewc, Morgantown, WV (US)
Assigned to Takeda Pharmaceutical Company Limited, Osaka (JP)
Appl. No. 16/62,734
Filed by Takeda Pharmaceutical Company Limited, Osaka (JP)
PCT Filed Dec. 15, 2016, PCT No. PCT/US2016/066936
§ 371(c)(1), (2) Date Jun. 15, 2018,
PCT Pub. No. WO2017/106504, PCT Pub. Date Jun. 22, 2017.
Claims priority of provisional application 62/267,734, filed on Dec. 15, 2015.
Prior Publication US 2021/0025898 A1, Jan. 28, 2021
Int. Cl. G01N 33/68 (2006.01); G01N 30/72 (2006.01); G01N 30/88 (2006.01); G01N 30/02 (2006.01)
CPC G01N 33/6848 (2013.01) [G01N 30/7233 (2013.01); G01N 30/88 (2013.01); G01N 33/6893 (2013.01); G01N 2030/027 (2013.01); G01N 2030/8831 (2013.01); G01N 2800/224 (2013.01); G01N 2800/50 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A method for detecting cleaved high molecular weight kininogen (HMWK) in a sample, the method comprising:
(i) providing a sample suspected of containing HMWK,
(ii) contacting the sample with a protease to generate a plurality of digested peptides; and
(iii) measuring the level of a signature peptide indicative of the 46 kDa light chain or 56 kDa light chain of cleaved HWMK in the plurality of digested peptides, wherein the signature peptide indicative of the 46 kDa light chain is:
 
 (SEQ ID NO: 1)
 
 KHNLGHGH,
 
 
 
 (SEQ ID NO: 2)
 
 KHNLGHGHKHE;
 
 
 
 (SEQ ID NO: 3)
 
 KHNLGHGHK;
 
 or
 
 
 
 (SEQ ID NO: 4)
 
 KHNLGHGHKHER;
 or,
wherein the signature peptide indicative of the 56 kDa light chain is SSRIGE (SEQ ID NO: 5).