US 11,668,708 B2
Method for reducing measurement error in latex agglutination immunoassay
Kengo Fujimura, Tokyo (JP); Junichi Kondou, Tokyo (JP); and Mitsuaki Yamamoto, Tokyo (JP)
Assigned to SEKISUI MEDICAL CO., LTD., Tokyo (JP)
Appl. No. 16/651,872
Filed by SEKISUI MEDICAL CO., LTD., Tokyo (JP)
PCT Filed Sep. 29, 2017, PCT No. PCT/JP2017/035479
§ 371(c)(1), (2) Date Mar. 27, 2020,
PCT Pub. No. WO2019/064491, PCT Pub. Date Apr. 4, 2019.
Prior Publication US 2020/0232977 A1, Jul. 23, 2020
Int. Cl. G01N 33/53 (2006.01); G01N 33/543 (2006.01)
CPC G01N 33/543 (2013.01) [G01N 2458/00 (2013.01)] 13 Claims
 
1. A method for measuring an analyte in a chylous plasma or chylous serum by a latex agglutination immunoassay, comprising the following steps:
(1) bringing a chylous plasma or chylous serum containing chyle into contact with imidazole or an imidazole derivative, or a salt thereof in a liquid phase;
(2) adding latex particles carrying a substance having a specific affinity for the analyte to the liquid phase sequentially after the step (1); and
(3) measuring an agglutination reaction between the analyte and the latex particles after the step (2), without performing a cleaning or separation step during or after the step (2),
wherein the concentration of imidazole or an imidazole derivative, or a salt thereof in the step (3) of measuring the agglutination reaction of the latex particles is not less than 100 mM and not more than 375 mM.