	US 11,667,978 B2
	*Detecting Babesia* species nucleic acid in a sample**
Vanessa Bres, San Diego, CA (US); Deanna Self, Escondido, CA (US); and Jeffrey M. Linnen, Poway, CA (US)
Assigned to GEN-PROBE INCORPORATED, San Diego, CA (US)
Appl. No. 16/611,806
Filed by Gen-Probe Incorporated, San Diego, CA (US)
PCT Filed Jun. 6, 2018, PCT No. PCT/US2018/036214 § 371(c)(1), (2) Date Nov. 7, 2019, PCT Pub. No. WO2018/226798, PCT Pub. Date Dec. 13, 2018.
Claims priority of provisional application 62/520,793, filed on Jun. 16, 2017.
Claims priority of provisional application 62/516,530, filed on Jun. 7, 2017.
Prior Publication US 2021/0079485 A1, Mar. 18, 2021
Int. Cl. C07H 21/04 (2006.01); C12Q 1/68 (2018.01); C12Q 1/6893 (2018.01); C12Q 1/6806 (2018.01)

CPC C12Q 1/6893 (2013.01) [C12Q 1/6806 (2013.01); C12Q 2600/16 (2013.01); C12Q 2600/166 (2013.01)]

14 Claims

1. A method for specifically detecting Babesia species nucleic acid in a sample, said method comprising:

(1) contacting a sample, said sample suspected of containing Babesia species nucleic acid, with at least two oligomers for amplifying a target region of a Babesia species target nucleic acid, wherein the at least two amplification oligomers comprise:

(a) a first amplification oligomer comprising a first target-hybridizing sequence that consists of SEQ ID NO:8; and

(b) a second amplification oligomer comprising a second target-hybridizing sequence that consists of SEQ ID NO:34;

(2) performing an in vitro nucleic acid amplification reaction, wherein any Babesia target nucleic acid present in said sample is used as a template for generating an amplification product; and,

(3) detecting the presence or absence of the amplification product, thereby indicating the presence or absence of Babesia species target nucleic acid in said sample.