CPC C12Q 1/6874 (2013.01) [B01L 3/502707 (2013.01); C12Q 1/6806 (2013.01); B01L 2300/0819 (2013.01); B01L 2300/0877 (2013.01); B01L 2300/0887 (2013.01); B01L 2300/16 (2013.01); B01L 2300/165 (2013.01)] | 11 Claims |
1. A flow cell, comprising:
a substrate including a bonding region at its periphery;
a lid bonded to the bonding region of the substrate;
a spacer layer positioned between the substrate and the lid so that the spacer layer contacts the bonding region, bonds the substrate and lid together, and forms sidewalls of the flow cell, wherein the lid and the substrate and the spacer layer define a flow channel between the substrate and the lid, and wherein the lid bonded to the substrate at the bonding region forms a boundary of the flow channel;
an input port and an output port formed in the lid or the substrate;
surface chemistry positioned on at least a portion of a surface of the substrate in the flow channel, the surface chemistry including:
a functionalized polymer coating layer covalently bound to a chemical group on the surface; and
a primer grafted to the functionalized polymer coating layer; and
a water-soluble protective coating applied on the primer and the functionalized polymer coating layer.
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