CPC C12Q 1/6869 (2013.01) [C12N 15/1065 (2013.01); C12Q 1/6855 (2013.01); C12Q 1/686 (2013.01); C40B 20/04 (2013.01); C40B 40/06 (2013.01); C40B 50/06 (2013.01)] | 14 Claims |
1. A polymerase-free reaction mix for fragmenting double-stranded DNA, comprising:
(a) a nickase that nicks double-stranded DNA;
(b) a single-strand DNA nuclease; and
(c) a DNA ligase capable of sealing a nick within a DNA; and
(d) double-stranded DNA to be fragmented,
wherein and the reaction mix does not contain a DNA polymerase.
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