CPC C12Q 1/6816 (2013.01) | 20 Claims |
1. A method for identifying target ligands in an array comprising:
(a) obtaining a first and a second subpopulation of beads, wherein each bead comprises:
(i) a capture probe capable of specifically binding to a target ligand,
(ii) a first polynucleotide comprising a barcode, wherein the barcode is indicative of the capture probe, and
(iii) a second polynucleotide comprising an index, wherein each index of the first subpopulation of beads is different from each index of the second subpopulation of beads;
(b) specifically binding first target ligands to the capture probes of the first subpopulation of beads in a first reaction volume, and specifically binding second target ligands to the capture probes of the second subpopulation of beads in a second reaction volume; and
(c) distributing the first and the second subpopulations of beads comprising the specifically bound first and second target ligands on a surface of a substrate, thereby obtaining an array of beads;
(d) detecting in the array the specifically bound first and second target ligands of the first and the second subpopulations of beads;
(e) sequencing the barcodes in the array of beads;
(f) sequencing the indexes in the array of beads; and
(g) decoding the locations of beads in the array of beads comprising a detected target ligand in the array, thereby identifying target ligands in the array of beads.
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