US 11,667,951 B2
Concealing information present within nucleic acids
Sterling Sawaya, Boulder, CO (US)
Assigned to GENEINFOSEC, INC., Boulder, CO (US)
Appl. No. 16/344,004
Filed by GENEINFOSEC, INC., Boulder, CO (US)
PCT Filed Oct. 24, 2017, PCT No. PCT/US2017/058076
§ 371(c)(1), (2) Date Apr. 22, 2019,
PCT Pub. No. WO2018/081113, PCT Pub. Date May 3, 2018.
Claims priority of provisional application 62/411,998, filed on Oct. 24, 2016.
Prior Publication US 2019/0233877 A1, Aug. 1, 2019
Int. Cl. C12Q 1/6806 (2018.01); C40B 70/00 (2006.01); C40B 20/04 (2006.01); C12N 15/10 (2006.01); C12N 15/66 (2006.01)
CPC C12Q 1/6806 (2013.01) [C12N 15/10 (2013.01); C12N 15/66 (2013.01); C40B 70/00 (2013.01); C40B 20/04 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A method for concealing nucleic acids, the method comprising: (a) generating a set of barcode oligonucleotides;
(b) affixing a subset of barcode oligonucleotides from the set of step (a) to a 5′- or 3′-end of target nucleic acids to create a set of barcoded target nucleic acids;
(c) concealing the set of barcoded target nucleic acids by pooling the set of barcoded target nucleic acids with one or more barcoded decoy nucleic acids to create a mixture of barcoded target nucleic acids and barcoded decoy nucleic acids, where the barcoded decoy nucleic acids contain a unique subset of barcode oligonucleotides different from the subset affixed to the target nucleic acids;
(d) preparing a table listing the identity and sequence(s) of the subset of barcode oligonucleotides affixed to the target nucleic acids and the identity and sequences of the barcode oligonucleotides of the barcoded decoy nucleic acids; and
(e) securing the mixture of barcoded target nucleic acids and the table by sealing both in a container that indicates when a seal of the container is broken.