	US 11,667,946 B2
	Detection of beta-hemolytic pathogens
Rongji Sum, Singapore (SG); Muthukaruppan Swaminathan, Singapore (SG); and Ian Cheong, Singapore (SG)
Assigned to Temasek Life Sciences Laboratory Limited, Singapore (SG)
Filed by TEMASEK LIFE SCIENCES LABORATORY LIMITED, Singapore (SG)
Filed on Oct. 22, 2020, as Appl. No. 17/77,581.
Application 17/077,581 is a division of application No. 16/079,270, granted, now 10,844,418, previously published as PCT/SG2017/050081, filed on Feb. 23, 2017.
Claims priority of provisional application 62/300,372, filed on Feb. 26, 2016.
Prior Publication US 2021/0108246 A1, Apr. 15, 2021
Int. Cl. C12Q 1/04 (2006.01); C12Q 1/10 (2006.01); C12Q 1/14 (2006.01)

CPC C12Q 1/04 (2013.01) [C12Q 1/10 (2013.01); C12Q 1/14 (2013.01)]

14 Claims

1. A kit for detecting the presence of a beta-hemolytic pathogen in a sample, the kit comprising sterically-stabilized liposomes and a fluorophore, wherein the sterically-stabilized liposomes are lysed by a beta-hemolytic pathogen and are not lysed by an alpha-hemolytic pathogen or by a gamma-hemolytic pathogen;

wherein the sterically-stabilized liposomes are prepared from a mixture of:

(1) a membrane forming phospholipid selected from the group consisting of (a) a saturated phosphatidylcholine and (b) a synthetic phosphatidylcholine with saturated fatty acid tails selected from the group consisting of dimyristoyl-phosphatidylcholine, dipalmitoyl-phosphatidylcholine, and distearoyl-phosphatidylcholine;

(2) cholesterol; and

(3) a conjugate of polyethylene glycol (PEG), wherein the PEG has a molecular weight from 1,000 to 5,000, and wherein the PEG is conjugated to a molecule selected from the group consisting of distearoyl phosphatidylethanolamine (DSPE), dipalmitoyl phosphatidylethanolamine (DPPE) and dimyristoyl phosphatidylethanolamine (DMPE);

wherein the molar ratio of (1):(2) is in the range of 2:1 to 1:1 and (3) is present at 5% (mol/mol); and

wherein the fluorophore is separate from the sterically-stabilized liposomes and is encapsulated into said liposomes for detecting the presence of the beta-hemolytic pathogen in the sample.