US 11,667,940 B2
De novo polynucleotide synthesis with substrate-bound polymerase
Bichlien Nguyen, Seattle, WA (US); Jake Smith, Seattle, WA (US); and Karin Strauss, Seattle, WA (US)
Assigned to MICROSOFT TECHNOLOGY LICENSING, LLC, Redmond, WA (US)
Filed by MICROSOFT TECHNOLOGY LICENSING, LLC, Redmond, WA (US)
Filed on May 28, 2020, as Appl. No. 16/886,638.
Prior Publication US 2021/0371891 A1, Dec. 2, 2021
Int. Cl. C12P 19/34 (2006.01); B01J 19/00 (2006.01)
CPC C12P 19/34 (2013.01) [B01J 19/0046 (2013.01); B01J 2219/00596 (2013.01); B01J 2219/00722 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A solid substrate having polymerases and polynucleotides tethered to a surface, wherein:
the solid substrate comprises a microelectrode array, or
the polymerases are tethered to the surface by a linker.