	US 11,667,939 B2
	Method for producing methacrylyl-CoA
Eiji Sato, Kanagawa (JP); Fujio Yu, Kanagawa (JP); Eiji Nakajima, Kanagawa (JP); Michiko Yamazaki, Kanagawa (JP); and Wataru Mizunashi, Kanagawa (JP)
Assigned to Mitsubishi Chemical Corporation, Tokyo (JP)
Filed by Mitsubishi Chemical Corporation, Tokyo (JP)
Filed on Apr. 10, 2019, as Appl. No. 16/379,823.
Application 16/379,823 is a continuation of application No. 14/905,155, granted, now 10,323,264, previously published as PCT/JP2014/003934, filed on Jul. 25, 2014.
Claims priority of application No. 2013-160302 (JP), filed on Aug. 1, 2013.
Prior Publication US 2019/0256880 A1, Aug. 22, 2019
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 19/32 (2006.01); C12N 9/88 (2006.01); C12P 7/52 (2006.01)

CPC C12P 19/32 (2013.01) [C12N 9/88 (2013.01); C12P 7/52 (2013.01)]

10 Claims

1. A method for producing methacrylyl-CoA and converting methylacryl-CoA to methacrylic acid or methacrylate ester, the method comprising:

converting 3-hydroxyisobutyryl-CoA to methacrylyl-CoA in an aqueous medium in the presence of a dehydratase; and

converting methacrylyl-CoA to methacrylic acid or methacrylate ester,

wherein the dehydratase is:

(a) a protein having the amino acid sequence shown in SEQ ID NO: 1; or

(b) a protein having an amino acid sequence in which one or two amino acids are deleted from, substituted with, added to and/or inserted into the amino acid sequence shown in SEQ ID NO: 1 and possessing dehydratase activity.