	US 11,667,936 B2
	Modified polypeptide with attenuated activity of citrate synthase and method for producing L-amino acid using the same
Jaewon Jang, Suwon-si (KR); Kwang Woo Lee, Suwon-si (KR); Yong Uk Shin, Yongin-si (KR); and Imsang Lee, Yongin-si (KR)
Assigned to CJ CHEILJEDANG CORPORATION, Seoul (KR)
Filed by CJ CHEILJEDANG CORPORATION, Seoul (KR)
Filed on Oct. 6, 2022, as Appl. No. 17/938,508.
Application 17/938,508 is a division of application No. 16/470,030, granted, now 11,499,173, previously published as PCT/KR2019/001697, filed on Feb. 12, 2019.
Claims priority of application No. 10-2018-0017400 (KR), filed on Feb. 13, 2018.
Prior Publication US 2023/0046561 A1, Feb. 16, 2023
Int. Cl. C12P 13/08 (2006.01); C12N 1/20 (2006.01); C12N 9/10 (2006.01); C12P 13/06 (2006.01); C12P 13/12 (2006.01); C12R 1/15 (2006.01)

CPC C12P 13/08 (2013.01) [C12N 1/20 (2013.01); C12N 9/1025 (2013.01); C12P 13/06 (2013.01); C12P 13/12 (2013.01); C12R 2001/15 (2021.05); C12Y 203/03001 (2013.01)]

7 Claims

1. A method for producing an L-amino acid, comprising:

culturing a microorganism in a medium,

wherein the microorganism is a microorganism of the genus Corynebacterium comprising a modified polypeptide having citrate synthase activity, wherein the 241st amino acid in the amino acid sequence of SEQ ID NO: 1, asparagine, is substituted with another amino acid and wherein the modified polypeptide has attenuated citrate synthase activity compared to the unmodified polypeptide; and

recovering an L-amino acid from the cultured microorganism or medium.