CPC C12N 15/87 (2013.01) [C12N 13/00 (2013.01)] | 30 Claims |
1. A method for transforming or transfecting cells comprising:
preparing a payload;
loading the payload on reagent delivery substrates;
combining the loaded reagent delivery substrates with a sphere-packing composition of cells and lattice-forming beads in a medium;
allowing the lattice-forming beads to self-assemble into a lattice having interstitial regions within the medium, wherein the loaded reagent delivery substrates are sized to fit into the interstitial regions of the self-assembled lattice formed by the lattice-forming beads, and wherein the cells are retained in the interstitial regions of the self-assembled lattice formed by the lattice-forming beads;
triggering release of reagents from the loaded reagent delivery substrates; and
providing electrical pulses to the sphere-packing composition of cells, lattice-forming beads, and reagents.
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