US 11,667,932 B2
Electroporation modules and instrumentation
Christian Siltanen, Boulder, CO (US); and Megan Basila, Boulder, CO (US)
Assigned to Inscripta, Inc., Boulder, CO (US)
Filed by Inscripta, Inc., Boulder, CO (US)
Filed on Nov. 10, 2021, as Appl. No. 17/523,821.
Application 17/523,821 is a continuation of application No. 17/158,488, filed on Jan. 26, 2021, granted, now 11,225,674.
Claims priority of provisional application 62/966,088, filed on Jan. 27, 2020.
Prior Publication US 2022/0073949 A1, Mar. 10, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/87 (2006.01); C12N 13/00 (2006.01)
CPC C12N 15/87 (2013.01) [C12N 13/00 (2013.01)] 30 Claims
 
1. A method for transforming or transfecting cells comprising:
preparing a payload;
loading the payload on reagent delivery substrates;
combining the loaded reagent delivery substrates with a sphere-packing composition of cells and lattice-forming beads in a medium;
allowing the lattice-forming beads to self-assemble into a lattice having interstitial regions within the medium, wherein the loaded reagent delivery substrates are sized to fit into the interstitial regions of the self-assembled lattice formed by the lattice-forming beads, and wherein the cells are retained in the interstitial regions of the self-assembled lattice formed by the lattice-forming beads;
triggering release of reagents from the loaded reagent delivery substrates; and
providing electrical pulses to the sphere-packing composition of cells, lattice-forming beads, and reagents.