CPC C12N 15/111 (2013.01) [C12N 2310/11 (2013.01); C12N 2310/14 (2013.01); C12N 2310/321 (2013.01); C12N 2310/322 (2013.01); C12N 2310/3515 (2013.01); C12N 2310/3519 (2013.01); C12N 2310/533 (2013.01); C12N 2320/51 (2013.01)] | 20 Claims |
1. An isolated double stranded nucleic acid molecule comprising a guide strand and a passenger strand, wherein the passenger strand is 8-18 continuous nucleotides in length, wherein the guide strand is 16-28 nucleotides in length, wherein the double stranded nucleic acid molecule comprises a double stranded region and a single stranded region, wherein the double stranded region is 8-18 nucleotides in length and is connected through a cleavable chemical linker to the single stranded region, and wherein the single stranded region comprises at least six phosphorothioate modified nucleotides.
|