CPC C12N 15/1017 (2013.01) [C12Q 1/6806 (2013.01); C12Q 1/6886 (2013.01); C12Q 2600/158 (2013.01)] | 17 Claims |
1. A detection method comprising:
generating a soluble urine concentrate from a soluble urine fraction by ultrafiltration, wherein the volume of soluble urine concentrate is reduced at least 50% from the original urine volume; and
contacting the soluble urine concentrate with at least one set of primer pairs and at least one probe to detect the amount of RNA from each of heat shock 60 kDa protein 1 (HSPD1), inosine monophosphate dehydrogenase 2 (IMPDH2), PDZ and LEVI domain 5 (PDLIM5), and UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1), wherein the at least one probe is labeled with a detectable label.
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