	US 11,667,907 B2
	Method and apparatus for encoding cellular spatial position information
Stevan Bogdan Jovanovich, Livermore, CA (US); and Peter Wagner, Menlo Park, CA (US)
Assigned to SILICON VALLEY SCIENTIFIC, INC., Livermore, CA (US)
Filed by SILICON VALLEY SCIENTIFIC, INC., Livermore, CA (US)
Filed on Jul. 21, 2021, as Appl. No. 17/381,796.
Application 17/381,796 is a division of application No. 15/771,607, granted, now 11,111,487, previously published as PCT/US2059/000232, filed on Oct. 27, 2016.
Claims priority of provisional application 62/247,368, filed on Oct. 28, 2015.
Prior Publication US 2022/0033802 A1, Feb. 3, 2022
Int. Cl. C12N 15/10 (2006.01); C12Q 1/6806 (2018.01)

CPC C12N 15/1003 (2013.01) [C12N 15/1017 (2013.01); C12N 15/1093 (2013.01); C12Q 1/6806 (2013.01)]

21 Claims

1. A method comprising:

(a) collecting a plurality of microsamples from a biological specimen;

(b) moving the collected microsamples into a fluidic stream in a known order; and

(c) adding, to individual microsamples in the fluidic stream in known order, a nucleic acid comprising a barcode of known sequence, wherein addition of a nucleic acid comprising a barcode of known sequence to an individual microsample in the stream encodes the original spatial position of the individual microsample within the biological specimen.