	US 11,667,904 B2
	CRISPR-associated systems and components
David R. Cheng, Boston, MA (US); David A. Scott, Cambridge, MA (US); and Winston X. Yan, Boston, MA (US)
Assigned to ARBOR BIOTECHNOLOGIES, INC., Cambridge, MA (US)
Filed by ARBOR BIOTECHNOLOGIES, INC., Cambridge, MA (US)
Filed on Apr. 29, 2020, as Appl. No. 16/862,261.
Application 16/862,261 is a continuation of application No. PCT/US2019/032750, filed on May 16, 2019.
Claims priority of provisional application 62/672,489, filed on May 16, 2018.
Prior Publication US 2020/0299659 A1, Sep. 24, 2020
Int. Cl. C12N 9/22 (2006.01); C12N 15/113 (2010.01); A61K 31/7088 (2006.01); A61K 38/46 (2006.01); C12N 15/11 (2006.01); C12N 15/90 (2006.01); C12Q 1/6832 (2018.01); C12N 9/96 (2006.01)

CPC C12N 9/22 (2013.01) [A61K 31/7088 (2013.01); A61K 38/465 (2013.01); C12N 9/96 (2013.01); C12N 15/11 (2013.01); C12N 15/113 (2013.01); C12N 15/907 (2013.01); C12Q 1/6832 (2013.01); C07K 2319/80 (2013.01); C12N 2310/20 (2017.05); C12N 2310/531 (2013.01); C12N 2800/80 (2013.01)]

20 Claims

1. An engineered, non-naturally occurring Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)—Cas system comprising:

an RNA guide comprising a direct repeat sequence and a spacer sequence capable of hybridizing to a target nucleic acid; and

a CRISPR-associated protein, wherein the CRISPR-associated protein comprises an amino acid sequence that is at least 99% identical to the amino acid sequence of SEQ ID NO: 316, SEQ ID NO: 317, or SEQ ID NO: 318;

wherein the CRISPR-associated protein is capable of binding to the RNA guide and of targeting the target nucleic acid sequence complementary to the spacer sequence.