	US 11,667,891 B2
	Method of selectively differentiating undifferentiated macrophage into M1 macrophage
Hojeong Jeon, Seoul (KR); Youngmin Seo, Seoul (KR); Hyunseon Seo, Seoul (KR); Myoung-Ryul Ok, Seoul (KR); Yu Chan Kim, Seoul (KR); Hyun Kwang Seok, Seoul (KR); and Seung Ja Oh, Seoul (KR)
Assigned to KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY, Seoul (KR)
Filed by KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY, Seoul (KR)
Filed on Jun. 10, 2020, as Appl. No. 16/898,072.
Claims priority of application No. 10-2019-0077180 (KR), filed on Jun. 27, 2019.
Prior Publication US 2020/0407684 A1, Dec. 31, 2020
Int. Cl. C12N 5/0786 (2010.01); C12M 1/42 (2006.01); C12M 1/34 (2006.01); C12M 1/12 (2006.01); C12N 5/078 (2010.01)

CPC C12N 5/0645 (2013.01) [C12M 25/02 (2013.01); C12M 35/04 (2013.01); C12M 41/40 (2013.01); C12N 5/0643 (2013.01); C12N 2506/115 (2013.01); C12N 2521/00 (2013.01); C12N 2533/30 (2013.01)]

2 Claims

1. A method for selective differentiation of undifferentiated macrophages into M1 macrophages, the method comprising incubating the undifferentiated macrophages in an incubator under a pressurized environment;

wherein the pressurized environment is created by a transwell or a pressing plate which vertically descends from an upper portion of the incubator to the inside of the incubator to mechanically press the top surface of said undifferentiated macrophages at a pressure corresponding to atmospheric pressure of 1.5-5;

wherein the transwell comprises: a hollow chamber; and a membrane which is provided in an empty space of the chamber and is composed of a porous film;

wherein a hydrogel is attached to a bottom surface of the membrane or the pressing plate;

wherein the hydrogel is formed by hydration of alginate and a concentration of the alginate is 2%, and wherein the incubating the undifferentiated macrophages in the incubator under a pressurized environment is for at least 48 hours.