US 11,667,884 B2
Bead beating tube and method for extracting deoxyribonucleic acid and/or ribonucleic acid from microorganisms
Holger Klapproth, Freiburg (DE); and Nicolaas Smit, Welland (CA)
Assigned to Safeguard Biosystems Holdings Ltd., London (GB)
Appl. No. 16/487,769
Filed by SAFEGUARD BIOSYSTEMS HOLDINGS LTD., London (GB)
PCT Filed Jan. 29, 2018, PCT No. PCT/EP2018/052173
§ 371(c)(1), (2) Date Aug. 21, 2019,
PCT Pub. No. WO2018/138363, PCT Pub. Date Aug. 2, 2018.
Claims priority of provisional application 62/538,073, filed on Jul. 28, 2017.
Claims priority of application No. 2017051902 (EP), filed on Jan. 30, 2017.
Prior Publication US 2021/0284951 A1, Sep. 16, 2021
Int. Cl. C12N 1/00 (2006.01); C12N 1/06 (2006.01); B01L 3/00 (2006.01); C12N 15/10 (2006.01); G01N 1/28 (2006.01)
CPC C12N 1/066 (2013.01) [B01L 3/5082 (2013.01); C12N 15/1006 (2013.01); G01N 1/286 (2013.01); C12N 2509/10 (2013.01); G01N 2001/2866 (2013.01)] 25 Claims
OG exemplary drawing
 
1. A method for producing and recovering a lysate from cells contained in blood containing an anticoagulant, and analyzing one or more nucleic acids from the lysate, comprising:
a) agitating the blood within a bead beating system in the absence of any additive other than the anticoagulant, under conditions sufficient to lyse both a fungal pathogen and a bacterial pathogen and provide a lysate suitable for PCR amplification of DNA from the fungal pathogen, when present, and the bacterial pathogen, when present, said bead beating system comprising (i) a sample tube or blood collection tube and (ii) beads;
b) recovering the lysate from the sample tube or blood collection tube; and
c) analyzing one or more nucleic acids from the lysate, wherein the analyzing comprises PCR to amplify DNA from the fungal pathogen, when present, and DNA from the bacterial pathogen, when present.