CPC C07K 16/00 (2013.01) [A61K 47/68 (2017.08); A61K 47/6817 (2017.08); A61K 47/6889 (2017.08); C07K 1/086 (2013.01); C07K 1/1133 (2013.01); C07K 1/36 (2013.01); C07K 2317/52 (2013.01); C07K 2317/53 (2013.01)] | 14 Claims |
1. A method for selective activation of engineered cysteine residues of engineered cysteine antibodies, said method comprising:
(a) diafiltering a mixture of engineered cysteine antibodies and buffer, and
(b) adding a reducing agent to said mixture in a concentration and at a rate to activate a portion of available engineered cysteine residues while retaining at least 80% of all inter-chain disulfide bonds present in the engineered cysteine antibodies prior to (b); and
(c) maintaining diafiltration of said mixture during step (b);
to selectively activate the engineered cysteine residues.
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