	US 11,667,679 B2
	Streptavidin muteins and methods of using them
Thomas Schmidt, Adelebsen (DE)
Assigned to IBA LIFESCIENCES GMBH, Goettingen (DE)
Filed by IBA LIFESCIENCES GMBH, Goettingen (DE)
Filed on Nov. 6, 2020, as Appl. No. 17/92,037.
Application 17/092,037 is a continuation of application No. 16/047,831, filed on Jul. 27, 2018, granted, now 10,844,099.
Application 16/047,831 is a continuation of application No. 14/442,376, granted, now 10,065,996, issued on Sep. 4, 2018, previously published as PCT/EP2013/074070, filed on Nov. 18, 2013.
Claims priority of provisional application 61/727,283, filed on Nov. 16, 2012.
Claims priority of application No. 13181697 (EP), filed on Aug. 26, 2013.
Prior Publication US 2021/0139545 A1, May 13, 2021
Int. Cl. C07K 14/36 (2006.01); C12N 15/62 (2006.01); G01N 33/68 (2006.01); C07K 1/22 (2006.01)

CPC C07K 14/36 (2013.01) [C12N 15/62 (2013.01); G01N 33/68 (2013.01); C07K 1/22 (2013.01); C07K 2319/22 (2013.01); C07K 2319/70 (2013.01); G01N 2333/36 (2013.01)]

19 Claims

1. A mutein, selected from muteins of streptavidin, wherein the mutein

(a) contains at least two cysteine residues in the region of the amino acid positions 44 to 53 with reference to the amino acid sequence of wild type streptavidin as set forth at SEQ ID NO: 212 and

(b) has a higher binding affinity than

(i) a streptavidin mutein “1” (SEQ ID NO: 112) that comprises the amino acid sequence Val⁴⁴-Thr⁴⁵-Ala⁴⁶-Arg⁴⁷(SEQ ID NO: 98), or

(ii) wild type-streptavidin of which amino acid residues 14 to 139 are shown as SEQ ID NO: 212 for peptide ligands comprising the amino acid sequence Trp-Ser-His-Pro-Gln-Phe-Glu-Lys (SEQ ID NO: 100).