US 11,666,872 B2
Containers for agitation of liquid samples and methods of use thereof
Adam Suchocki, Lexington, MA (US); Mark John Audeh, Brighton, MA (US); Matthew Blanco, Brookline, MA (US); James Franklin Chepin, San Diego, CA (US); Vasiliki Demas, Arlington, MA (US); Marilyn Lee Fritzemeier, Lexington, MA (US); Thomas Jay Lowery, Jr., Belmont, MA (US); Michael Min, Boston, MA (US); Lori Anne Neely, Reading, MA (US); Charles William Rittershaus, Malden, MA (US); Hwa-Tang Wang, Lexington, MA (US); and Parris Wellman, Reading, MA (US)
Assigned to T2 Biosystems, Inc., Lexington, MA (US)
Filed by T2 Biosystems, Inc., Lexington, MA (US)
Filed on Oct. 21, 2019, as Appl. No. 16/659,215.
Application 15/453,356 is a division of application No. 13/650,734, filed on Oct. 12, 2012, granted, now 9,707,528, issued on Jul. 18, 2017.
Application 16/659,215 is a continuation of application No. 15/453,356, filed on Mar. 8, 2017, granted, now 10,486,118.
Claims priority of provisional application 61/601,842, filed on Feb. 22, 2012.
Prior Publication US 2020/0261873 A1, Aug. 20, 2020
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 1/06 (2006.01); B01F 29/30 (2022.01); G01N 27/74 (2006.01); G01N 33/49 (2006.01); C12M 1/00 (2006.01); B65D 1/46 (2006.01); C12Q 1/6816 (2018.01); C12Q 1/686 (2018.01); B01F 33/25 (2022.01); B01F 35/53 (2022.01)
CPC B01F 29/30 (2022.01) [B01F 33/251 (2022.01); B01F 35/53 (2022.01); B01F 35/5312 (2022.01); B65D 1/46 (2013.01); C12M 47/06 (2013.01); C12N 1/066 (2013.01); C12Q 1/686 (2013.01); C12Q 1/6816 (2013.01); G01N 27/745 (2013.01); G01N 33/492 (2013.01); B01F 2215/0431 (2013.01); B65D 2221/00 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A method of lysing cells in a liquid sample, said method comprising:
(a) providing liquid in a container comprising: (i) an interior chamber with a central axis, (ii) a top comprising an opening, and (iii) a substantially circular cross section or a substantially polygonal cross section, wherein the surface of said interior chamber comprises one or more substantially linear protrusions substantially parallel to said central axis, and wherein said liquid comprises rigid particles and cells; and
(b) agitating said liquid in said container,
wherein said agitation is of a sufficient force and duration to lyse said cells.