US 11,666,084 B2
Tobacco-derived colorants and colored substrates
Charles Stewart, Kingsport, TN (US); Ercilia Hernandez Garcia, Clayton, NC (US); and Robert Owen Bussey, III, Winston-Salem, NC (US)
Assigned to R.J. Reynolds Tobacco Company, Winston-Salem, NC (US)
Filed by R.J. Reynolds Tobacco Company, Winston-Salem, NC (US)
Filed on Jun. 25, 2020, as Appl. No. 16/912,257.
Application 16/912,257 is a division of application No. 15/284,704, filed on Oct. 4, 2016, granted, now 10,721,957.
Prior Publication US 2020/0323256 A1, Oct. 15, 2020
Int. Cl. A24B 15/30 (2006.01); C09B 61/00 (2006.01); D06P 1/34 (2006.01); D06P 3/14 (2006.01); D06P 3/60 (2006.01); D06P 5/15 (2006.01); D06P 1/00 (2006.01)
CPC A24B 15/307 (2013.01) [C09B 61/00 (2013.01); D06P 1/0016 (2013.01); D06P 1/34 (2013.01); D06P 3/14 (2013.01); D06P 3/60 (2013.01); D06P 5/158 (2013.01)] 21 Claims
OG exemplary drawing
 
1. A colored substrate prepared according to a method comprising:
providing a first tobacco mixture by combining a tobacco material with a cellulase and a beta glucosidase at a pH of about 5-6;
providing a second tobacco mixture by adjusting the pH of the first tobacco mixture to a pH of about 7-8 and adding pectate lyase and xylanase to the first tobacco mixture;
separating the tobacco material from the second tobacco mixture to give a colorant-containing broth; and
contacting a substrate with the colorant-containing broth to give the colored substrate,
wherein the colored substrate exhibits color that is independent of lighting.