CPC A24B 15/307 (2013.01) [C09B 61/00 (2013.01); D06P 1/0016 (2013.01); D06P 1/34 (2013.01); D06P 3/14 (2013.01); D06P 3/60 (2013.01); D06P 5/158 (2013.01)] | 21 Claims |
1. A colored substrate prepared according to a method comprising:
providing a first tobacco mixture by combining a tobacco material with a cellulase and a beta glucosidase at a pH of about 5-6;
providing a second tobacco mixture by adjusting the pH of the first tobacco mixture to a pH of about 7-8 and adding pectate lyase and xylanase to the first tobacco mixture;
separating the tobacco material from the second tobacco mixture to give a colorant-containing broth; and
contacting a substrate with the colorant-containing broth to give the colored substrate,
wherein the colored substrate exhibits color that is independent of lighting.
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