US 11,656,447 B2
Apparatus and method for fast volumetric fluorescence microscopy using temporally multiplexed light sheets
Kevin Kin Man Tsia, Hong Kong (CN); Yuxuan Ren, Hong Kong (CN); Jianglai Wu, Hong Kong (CN); Andy Kam Seng Lau, Hong Kong (CN); and Queenie Tsz Kwan Lai, Hong Kong (CN)
Assigned to THE UNIVERSITY OF HONG KONG, Hong Kong (CN)
Appl. No. 17/267,567
Filed by THE UNIVERSITY OF HONG KONG, Hong Kong (CN)
PCT Filed Sep. 27, 2019, PCT No. PCT/CN2019/108625
§ 371(c)(1), (2) Date Feb. 10, 2021,
PCT Pub. No. WO2020/063895, PCT Pub. Date Apr. 2, 2020.
Claims priority of provisional application 62/738,261, filed on Sep. 28, 2018.
Prior Publication US 2021/0325651 A1, Oct. 21, 2021
Int. Cl. G02B 21/00 (2006.01); G01N 21/64 (2006.01); G01N 21/88 (2006.01); G02B 21/36 (2006.01)
CPC G02B 21/0076 (2013.01) [G01N 21/6458 (2013.01); G01N 21/6486 (2013.01); G01N 21/8851 (2013.01); G02B 21/0032 (2013.01); G02B 21/367 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A light-sheet based microscopy device comprising:
a light source;
a pair of mirrors configured to receive light from the light source and generate an array of coherent light sheets, wherein the coherent light sheets are mutually spatially incoherent from each other, and wherein the pair of mirrors is further configured to reconfigure a coherency of the coherent light sheets with respect to each other;
an encoder configured to encode the array of incoherent light sheets, the encoder comprising: a frequency modulator configured to segment the array of coherent light sheets and encode each coherent light sheet with a respective frequency; or a temporal modulator configured to segment the array of coherent light sheets by an arbitrary orthogonal encoding basis including a Hadamard basis or a random mask;
at least one lens configured to direct the encoded light sheets towards a biological sample, and
an image capturing device configured to receive a fluorescence signal from the biological sample.