US 11,656,176 B2
Near-infrared spectroscopy-based method for chemical pattern recognition of authenticity of traditional Chinese medicine Gleditsiae spina
Tiejie Wang, Shenzhen Guangdong (CN); Lijun Wang, Guangdong (CN); Yan Yan, Guangdong (CN); Jue Wang, Guangdong (CN); Guo Yin, Guangdong (CN); Kun Jiang, Guangdong (CN); Jing Li, Guangdong (CN); Yang Wang, Guangdong (CN); and Yin Hui, Guangdong (CN)
Assigned to SHENZHEN INSTITUTE FOR DRUG CONTROL (SHENZHEN TESTING CENTER OF MEDICAL DEVICES)
Appl. No. 17/43,325
Filed by SHENZHEN INSTITUTE FOR DRUG CONTROL (SHENZHEN TESTING CENTER OF MEDICAL DEVICES), Guangdong (CN)
PCT Filed Apr. 1, 2019, PCT No. PCT/CN2019/080873
§ 371(c)(1), (2) Date Sep. 29, 2020,
PCT Pub. No. WO2019/192433, PCT Pub. Date Oct. 10, 2019.
Claims priority of application No. 201810290323.7 (CN), filed on Apr. 3, 2018.
Prior Publication US 2021/0025815 A1, Jan. 28, 2021
Int. Cl. G01N 21/359 (2014.01); G06F 18/23 (2023.01); G06F 18/2115 (2023.01); G06F 18/2132 (2023.01); G01N 21/3504 (2014.01); G01N 21/3563 (2014.01); G01N 21/3577 (2014.01)
CPC G01N 21/359 (2013.01) [G06F 18/2115 (2023.01); G06F 18/2132 (2023.01); G06F 18/23 (2023.01); G01N 21/3504 (2013.01); G01N 21/3563 (2013.01); G01N 21/3577 (2013.01); G01N 2201/1296 (2013.01)] 10 Claims
OG exemplary drawing
 
1. A near-infrared spectroscopy-based method for chemical pattern recognition of authenticity of a traditional Chinese medicine, Gleditsiae Spina, comprising:
(1) collecting near-infrared spectrums of samples of the Gleditsiae Spina and counterfeits thereof, removing an internal reference background, and collecting spectrums at three different positions on a surface of each of the samples to obtain an average spectrum as an original spectrum;
(2) removing interference peaks in the original spectrum to obtain peaks within spectral bands of 11800-7500 cm−1, 6500-5500 cm−1, and 5000-4200 cm−1, selecting peaks within the spectral band of 5000-4200 cm−1 as model analysis peaks, and pre-treating the peaks within the spectral band of 5000-4200 cm−1 by using a first derivative (1st D) pre-treatment method;
(3) screening characteristic wave number points within a range of 5000-4200 cm-1 subjected to the 1st D pre-treatment method by using a successive projections algorithm, and dividing the Gleditsiae Spina and the counterfeits thereof to be discriminated according to the characteristic wave number points by using a Kennard-Stone algorithm into samples in a training set and samples in a test set;
(4) establishing a discriminant model based on the samples in the training set, extracting a total of five characteristic wave number points, x8, x13, x16, x19, and x21, by using a step-by-step method, and applying the five characteristic wave number points to establish the following discriminant functions:
F1=36387.907x8+24242.533x13+9262.246x16+11456.025x19+13209.943x21+3.210, and
F2=−43757.506x8+40701.987x13+24623.897x16+28906.269x19−20234.651x21+4.496; and
(5) substituting the total of five characteristic wave number points, x8, x13, x16, x19, and x21, of the samples in the test set into the discriminant functions obtained in step (4) to discriminate discriminant accuracies of the Gleditsiae Spina and the counterfeits thereof.