	US 11,655,489 B2
	Method for improving n-3 polyunsaturated fatty acids enrichment efficiency in glyceride
Xiaosan Wang, Wuxi (CN); Ye Chen, Wuxi (CN); Cong Jiang, Wuxi (CN); Zhuangzhuang Yang, Wuxi (CN); Yifan Wang, Wuxi (CN); and Wenhua Jin, Wuxi (CN)
Assigned to Jiangnan University, Wuxi (CN)
Filed by Jiangnan University, Wuxi (CN)
Filed on Aug. 25, 2021, as Appl. No. 17/411,076.
Prior Publication US 2022/0119852 A1, Apr. 21, 2022
Int. Cl. C12P 7/6472 (2022.01)

CPC C12P 7/6472 (2013.01)

8 Claims

1. A method for improving eicosapentaenoic acid (EPA) and/or docosahexaenoic acid (DHA) glyceride enrichment efficiency, the method comprising:

providing an oil, a lipase, metal ions and an aqueous solution in a reaction system, wherein the oil comprises EPA and/or DHA;

obtaining a mixture of the oil, lipase, metal ions and aqueous solution after a reaction time in the reaction system at pH 5-8 and at 20-50° C.; and

removing free fatty acid from the mixture to obtain a EPA and/or DHA-rich glyceride,

wherein the lipase is selected from Candida cylindracea lipase, Rhizopus oryzea lipase, Candida antarctica lipase A of Candida antarctica, or one or more of Aspergillus sp. lipase,

wherein the metal ions comprising one or more of calcium ions, magnesium ions, manganese ions (2+) and ferric ions (3+), and

wherein the aqueous solution comprises one or more of pure water solution, phosphate buffered solution and citrate buffered solution.