US 11,655,482 B2
Recombinant transition vector for increasing foreign protein expression
Soo Dong Woo, Chungcheongbuk-do (KR); Ji Hoon Lee, Gyeonggi-do (KR); Won Seok Gwak, Chungcheongbuk-do (KR); Ji In Ma, Gyeonggi-do (KR); Beom Ku Han, Daejeon (KR); Hyun Il Kim, Seoul (KR); Charl Se Park, Chungcheongbuk-do (KR); and Jae Bang Choi, Chungcheongbuk-do (KR)
Assigned to OPTIPHARM CO., LTD, Chungcheongbuk-Do (KR); and CHUNGBUK NATIONAL UNIVERSITY INDUSTRY ACADEMIC COOPERATION FOUNDATION, Chungcheongbuk-Do (KR)
Appl. No. 16/645,386
Filed by OPTIPHARM CO., LTD, Chungcheongbuk-do (KR); and CHUNGBUK NATIONAL UNIVERSITY INDUSTRY ACADEMIC COOPERATION FOUNDATION, Chungcheongbuk-do (KR)
PCT Filed Mar. 7, 2018, PCT No. PCT/KR2018/002716
§ 371(c)(1), (2) Date Mar. 6, 2020,
PCT Pub. No. WO2019/050111, PCT Pub. Date Mar. 14, 2019.
Claims priority of application No. 10-2017-0114614 (KR), filed on Sep. 7, 2017.
Prior Publication US 2020/0291426 A1, Sep. 17, 2020
Int. Cl. C12N 15/86 (2006.01); C12N 7/00 (2006.01); C12P 21/00 (2006.01)
CPC C12N 15/86 (2013.01) [C12N 7/00 (2013.01); C12P 21/00 (2013.01); C12N 2710/14021 (2013.01); C12N 2710/14043 (2013.01)] 9 Claims
 
1. A recombinant transition vector comprising:
a) a promoter of a polyhedrin protein coding gene of baculovirus;
b) a burst sequence represented by SEQ ID NO: 2 and operably linked to the promoter; and
c) a hr5 (homologous region 5) sequence represented by SEQ ID NO: 1;
wherein the hr5 sequence represented by SEQ ID NO: 1, the promoter of the polyhedrin protein coding gene of baculovirus, and the burst sequence represented by SEQ ID NO: 2 are sequentially linked to each other.