CPC C12N 15/1013 (2013.01) [B01L 3/502 (2013.01); B01L 3/508 (2013.01); B01L 3/5027 (2013.01); B01L 3/50273 (2013.01); B01L 3/502707 (2013.01); B01L 3/502715 (2013.01); B01L 3/502723 (2013.01); B01L 3/502738 (2013.01); B01L 3/502761 (2013.01); B01L 3/527 (2013.01); B01L 3/567 (2013.01); B01L 7/52 (2013.01); B01L 7/525 (2013.01); C12M 23/42 (2013.01); C12Q 1/68 (2013.01); C12Q 1/686 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6813 (2013.01); B01L 3/5025 (2013.01); B01L 3/502746 (2013.01); B01L 2200/025 (2013.01); B01L 2200/027 (2013.01); B01L 2200/0605 (2013.01); B01L 2200/0615 (2013.01); B01L 2200/0668 (2013.01); B01L 2200/0684 (2013.01); B01L 2200/0689 (2013.01); B01L 2200/10 (2013.01); B01L 2200/12 (2013.01); B01L 2200/142 (2013.01); B01L 2300/021 (2013.01); B01L 2300/022 (2013.01); B01L 2300/044 (2013.01); B01L 2300/06 (2013.01); B01L 2300/0609 (2013.01); B01L 2300/0627 (2013.01); B01L 2300/0636 (2013.01); B01L 2300/0672 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/087 (2013.01); B01L 2300/0809 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0861 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/0867 (2013.01); B01L 2300/0883 (2013.01); B01L 2300/0887 (2013.01); B01L 2300/0893 (2013.01); B01L 2300/123 (2013.01); B01L 2300/14 (2013.01); B01L 2300/16 (2013.01); B01L 2300/1805 (2013.01); B01L 2300/1822 (2013.01); B01L 2300/1827 (2013.01); B01L 2400/043 (2013.01); B01L 2400/0406 (2013.01); B01L 2400/0478 (2013.01); B01L 2400/0481 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/065 (2013.01); B01L 2400/0622 (2013.01); B01L 2400/0655 (2013.01); B01L 2400/0694 (2013.01); B01L 2400/086 (2013.01); B29C 65/08 (2013.01); B29C 65/484 (2013.01); B29C 65/606 (2013.01); B29C 66/71 (2013.01); B29C 66/81423 (2013.01); B29C 66/8322 (2013.01); B29L 2031/756 (2013.01); C12N 13/00 (2013.01)] | 11 Claims |
1. A molecular diagnostic system configured to process a biological sample within a cartridge and separate a nucleic acid component from the biological sample, the molecular diagnostic system comprising:
a translatable cartridge platform located at least partially within the molecular diagnostic system and configured to receive and align a cartridge comprising:
a rigid top layer defining a central region, a heating region at the central region, and a downstream end region; and
a fluidic pathway defining a flow path through the heating region and to a detection chamber at the downstream end region, the detection chamber comprising a surface exposed by the translatable cartridge platform;
a detection chamber heater vertically aligned with the surface of the detection chamber, inferior to the translatable cartridge platform;
an actuator aligned with the translatable cartridge platform and coupled to a cartridge heater aligned with the heating region across a portion of the flow path of the cartridge,
wherein extension of the actuator compresses the cartridge heater against the heating region of the cartridge and the cartridge against the translatable cartridge platform, thereby vertically translating the translatable cartridge platform, with the cartridge, from a baseline position to a compressed mode and establishing communication between the detection chamber heater and the detection chamber; and
a set of springs coupled to the translatable cartridge platform at positions opposing the actuator, such that the translatable cartridge platform is biased towards the baseline position.
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