US 11,655,467 B2
System and method for processing and detecting nucleic acids
Jeffrey Williams, Ann Arbor, MI (US); Sundaresh Brahmasandra, Ann Arbor, MI (US); and Michael T. Kusner, Ann Arbor, MI (US)
Assigned to NeuMoDx Molecular, Inc., Ann Arbor, MI (US)
Filed by NeuMoDx Molecular, Inc., Ann Arbor, MI (US)
Filed on Aug. 29, 2016, as Appl. No. 15/249,771.
Application 15/249,771 is a continuation of application No. 15/134,765, filed on Apr. 21, 2016, granted, now 10,041,062.
Application 15/134,765 is a continuation of application No. 14/704,215, filed on May 5, 2015, granted, now 9,441,219, issued on Sep. 13, 2016.
Application 14/704,215 is a continuation of application No. 13/766,359, filed on Feb. 13, 2013, granted, now 9,050,594, issued on Jun. 9, 2015.
Application 13/766,359 is a continuation of application No. 13/765,996, filed on Feb. 13, 2013, granted, now 9,738,887.
Claims priority of provisional application 61/667,606, filed on Jul. 3, 2012.
Claims priority of provisional application 61/598,240, filed on Feb. 13, 2012.
Prior Publication US 2016/0362677 A1, Dec. 15, 2016
Int. Cl. C12N 15/10 (2006.01); B01L 3/00 (2006.01); B01L 99/00 (2010.01); C12M 3/00 (2006.01); C12Q 1/68 (2018.01); C12Q 1/6806 (2018.01); B01L 7/00 (2006.01); C12Q 1/686 (2018.01); C12Q 1/6813 (2018.01); C12N 13/00 (2006.01); B29C 65/08 (2006.01); B29C 65/60 (2006.01); B29C 65/00 (2006.01); B29L 31/00 (2006.01); B29C 65/48 (2006.01)
CPC C12N 15/1013 (2013.01) [B01L 3/502 (2013.01); B01L 3/508 (2013.01); B01L 3/5027 (2013.01); B01L 3/50273 (2013.01); B01L 3/502707 (2013.01); B01L 3/502715 (2013.01); B01L 3/502723 (2013.01); B01L 3/502738 (2013.01); B01L 3/502761 (2013.01); B01L 3/527 (2013.01); B01L 3/567 (2013.01); B01L 7/52 (2013.01); B01L 7/525 (2013.01); C12M 23/42 (2013.01); C12Q 1/68 (2013.01); C12Q 1/686 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6813 (2013.01); B01L 3/5025 (2013.01); B01L 3/502746 (2013.01); B01L 2200/025 (2013.01); B01L 2200/027 (2013.01); B01L 2200/0605 (2013.01); B01L 2200/0615 (2013.01); B01L 2200/0668 (2013.01); B01L 2200/0684 (2013.01); B01L 2200/0689 (2013.01); B01L 2200/10 (2013.01); B01L 2200/12 (2013.01); B01L 2200/142 (2013.01); B01L 2300/021 (2013.01); B01L 2300/022 (2013.01); B01L 2300/044 (2013.01); B01L 2300/06 (2013.01); B01L 2300/0609 (2013.01); B01L 2300/0627 (2013.01); B01L 2300/0636 (2013.01); B01L 2300/0672 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/087 (2013.01); B01L 2300/0809 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0861 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/0867 (2013.01); B01L 2300/0883 (2013.01); B01L 2300/0887 (2013.01); B01L 2300/0893 (2013.01); B01L 2300/123 (2013.01); B01L 2300/14 (2013.01); B01L 2300/16 (2013.01); B01L 2300/1805 (2013.01); B01L 2300/1822 (2013.01); B01L 2300/1827 (2013.01); B01L 2400/043 (2013.01); B01L 2400/0406 (2013.01); B01L 2400/0478 (2013.01); B01L 2400/0481 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/065 (2013.01); B01L 2400/0622 (2013.01); B01L 2400/0655 (2013.01); B01L 2400/0694 (2013.01); B01L 2400/086 (2013.01); B29C 65/08 (2013.01); B29C 65/484 (2013.01); B29C 65/606 (2013.01); B29C 66/71 (2013.01); B29C 66/81423 (2013.01); B29C 66/8322 (2013.01); B29L 2031/756 (2013.01); C12N 13/00 (2013.01)] 11 Claims
OG exemplary drawing
 
1. A molecular diagnostic system configured to process a biological sample within a cartridge and separate a nucleic acid component from the biological sample, the molecular diagnostic system comprising:
a translatable cartridge platform located at least partially within the molecular diagnostic system and configured to receive and align a cartridge comprising:
a rigid top layer defining a central region, a heating region at the central region, and a downstream end region; and
a fluidic pathway defining a flow path through the heating region and to a detection chamber at the downstream end region, the detection chamber comprising a surface exposed by the translatable cartridge platform;
a detection chamber heater vertically aligned with the surface of the detection chamber, inferior to the translatable cartridge platform;
an actuator aligned with the translatable cartridge platform and coupled to a cartridge heater aligned with the heating region across a portion of the flow path of the cartridge,
wherein extension of the actuator compresses the cartridge heater against the heating region of the cartridge and the cartridge against the translatable cartridge platform, thereby vertically translating the translatable cartridge platform, with the cartridge, from a baseline position to a compressed mode and establishing communication between the detection chamber heater and the detection chamber; and
a set of springs coupled to the translatable cartridge platform at positions opposing the actuator, such that the translatable cartridge platform is biased towards the baseline position.