	US 11,655,464 B2
	Alkaline protease mutant, and gene, engineered strain, preparation method and application thereof
Fuping Lu, Tianjin (CN); Yihan Liu, Tianjin (CN); Yu Li, Tianjin (CN); Xingji Wang, Linyi (CN); Kefen Wang, Linyi (CN); Wenlong Liu, Linyi (CN); Fufeng Liu, Tianjin (CN); and Huitu Zhang, Tianjin (CN)
Assigned to Tianjin University of Science and Technology, Tianjin (CN); and Shandong Lonct Enzymes Co. LTD, Linyi (CN)
Filed by Tianjin University of Science and Technology, Tianjin (CN); and Shandong Lonct Enzymes Co. LTD, Linyi (CN)
Filed on Jul. 31, 2022, as Appl. No. 17/878,040.
Application 17/878,040 is a continuation of application No. PCT/CN2021/071281, filed on Jan. 12, 2021.
Claims priority of application No. 202011513325.1 (CN), filed on Dec. 21, 2020.
Prior Publication US 2023/0016224 A1, Jan. 19, 2023
Int. Cl. C12N 9/54 (2006.01); C12N 1/21 (2006.01); C12N 15/55 (2006.01); C12N 15/75 (2006.01)

CPC C12N 9/54 (2013.01)

9 Claims

1. An alkaline protease mutant comprising an amino acid sequence having at least 75% identity to SEQ ID NO: 6 and having any one of the following mutation combinations:

V11I/G23A/G25P/135V/G95P/S99H/V145I/N212S/A267G,

V11L/G23A/G25P/135V/G95P/S99H/V145I/N212S/A267G,

V11L/G23A/G25A/135V/G95P/S99H/V145I/N212S/A267G,

V11I/G23A/G25A/135V/G95P/S99H/V145I/N212S/A267G,

V11I/G23A/G25A/135V/G95P/S99H/V145I/N212S/A267P,

V11L/G23A/G25A/135V/G95P/S99H/V145I/N212S/A267P,

V11I/G23A/G25P/135V/G95P/S99H/V145I/N212S/A267P, or

V11L/G23A/G25P/135V/G95P/S99H/V145I/N212S/A267P.