US 11,655,447 B2
Global gene regulators (GGR) as vaccine candidates against paratuberculosis
Adel M. Talaat, Madison, WI (US)
Assigned to WISCONSIN ALUMNI RESEARCH FOUNDATION, Madison, WI (US)
Filed by Wisconsin Alumni Research Foundation, Madison, WI (US)
Filed on Apr. 2, 2021, as Appl. No. 17/221,605.
Application 16/718,892 is a division of application No. 15/492,553, filed on Apr. 20, 2017, granted, now 10,544,389, issued on Jan. 28, 2020.
Application 17/221,605 is a continuation of application No. 16/718,892, filed on Dec. 18, 2019, granted, now 10,968,425.
Application 15/492,553 is a continuation of application No. 14/193,818, filed on Feb. 28, 2014, granted, now 9,663,758, issued on May 30, 2017.
Claims priority of provisional application 61/777,907, filed on Mar. 12, 2013.
Prior Publication US 2021/0246416 A1, Aug. 12, 2021
Int. Cl. C12N 1/20 (2006.01); C12Q 1/689 (2018.01); C07K 14/195 (2006.01); C07K 14/35 (2006.01); C12N 1/36 (2006.01); G01N 33/569 (2006.01); A61K 39/04 (2006.01); C12N 15/10 (2006.01); C12N 15/113 (2010.01); A61K 39/00 (2006.01); C12R 1/32 (2006.01)
CPC C12N 1/20 (2013.01) [A61K 39/04 (2013.01); C07K 14/195 (2013.01); C07K 14/35 (2013.01); C12N 1/36 (2013.01); C12N 15/1031 (2013.01); C12N 15/1136 (2013.01); C12Q 1/689 (2013.01); G01N 33/5695 (2013.01); A61K 2039/522 (2013.01); A61K 2039/552 (2013.01); C12N 1/205 (2021.05); C12Q 2600/156 (2013.01); C12R 2001/32 (2021.05)] 22 Claims
 
1. A Mycobacterium mutant, comprising a mutation in a gene sequence encoding global gene regulators (GGRs), wherein the GGR is sigE, wherein the GGR gene is at least partially inactivated, wherein the Mycobacterium is selected from the group consisting of Mycobacterium avium subspecies paratuberculosis (M. ap) and Mycobacterium bovis (M. bovis).