CPC C07K 14/55 (2013.01) [A61K 47/60 (2017.08); A61K 47/62 (2017.08); C07K 14/5406 (2013.01); C07K 14/5437 (2013.01); C07K 14/5443 (2013.01); A61K 38/00 (2013.01); C07B 2200/13 (2013.01); C07K 2319/30 (2013.01); C07K 2319/33 (2013.01); C07K 2319/74 (2013.01)] | 20 Claims |
1. A non-naturally occurring polypeptide comprising domains X1, X2, X3, and X4, wherein:
(a) X1 is a peptide comprising an amino acid sequence at least 80% identical to the amino acid sequence PKKKIQLHAEHALYDALMILNI (SEQ ID NO: 4);
(b) X2 is a helical-peptide of at least 8 amino acids in length that has no binding site for the IL-2 receptor α subunit (IL-2Rα);
(c) X3 is a peptide comprising an amino acid sequence at least 80% identical to the amino acid sequence LEDYAFNFELILEEIARLFESG (SEQ ID NO:5); and
(d) X4 is a peptide comprising an amino acid sequence at least 80% identical to the amino acid sequence EDEQEEMANAIITILQSWIFS (SEQ ID NO:6);
wherein the domains are arranged N-terminal to C-terminal in an arrangement X1-X3-X2-X4;
wherein amino acid linkers may be present between any of the domains; and
wherein the polypeptide binds to the IL-2 receptor βc heterodimer (IL-2Rβc).
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