US 11,654,177 B2
Aloe extract and methods for producing the same
Thomas Chen, Taipei (TW); Chih-Sheng Hung, Taipei (TW); Lung-Yao Wang, Taipei (TW); and Yi-Ru Chen, Taipei (TW)
Assigned to DAZZEON BIOTECHNOLOGY CO., LTD., New Taipei (TW)
Filed by DAZZEON BIOTECHNOLOGY CO., LTD., New Taipei (TW)
Filed on Sep. 6, 2021, as Appl. No. 17/467,444.
Claims priority of application No. 109130748 (TW), filed on Sep. 8, 2020.
Prior Publication US 2022/0072080 A1, Mar. 10, 2022
Int. Cl. A61K 36/886 (2006.01)
CPC A61K 36/886 (2013.01) [A61K 2236/15 (2013.01); A61K 2236/331 (2013.01); A61K 2236/333 (2013.01); A61K 2236/39 (2013.01); A61K 2236/51 (2013.01); A61K 2236/53 (2013.01)] 11 Claims
 
1. A method for preparing an aloe extract, comprising:
(a) mincing a mesophyll of an Aloe in water;
(b) heating the minced mixture of the step (a) to 60-80° C.;
(c) filtering the mixture of the step (b) to produce a first filtrate;
(d) drying the first filtrate of the step (c);
(e) reconstituting the dried first filtrate of the step (d) in water;
(f) subjecting the first filtrate of the step (c) or the reconstituent of the step (e) to column chromatography and eluting the column packed with non-polar resins therein with a first polar solvent;
(g) concentrating the eluent of the step (f);
(h) purifying the concentrate of the step (g) with active carbons;
(i) filtering the purified concentrate of the step (h) to produce a second filtrate thereof;
(j) concentrating the second filtrate of the step (i); and
(k) adding a second polar solvent to the concentrated second filtrate of the step (j) thereby producing the aloe extract;
wherein the aloe extract has a level of O-acetyl groups above 200,000 mg/kg, a level of polysaccharides above 100,000 mg/kg; or a water solubility of at least 10 mg/ml.