	US 11,965,218 B2
	Methods for detecting respiratory viral infection
Ellen Foxman, West Hartford, CT (US); and Marie Landry, Guilford, CT (US)
Assigned to YALE UNIVERSITY, New Haven, CT (US)
Appl. No. 16/340,328
Filed by Yale University, New Haven, CT (US)
PCT Filed Oct. 11, 2017, PCT No. PCT/US2017/056076 § 371(c)(1), (2) Date Apr. 8, 2019, PCT Pub. No. WO2018/071498, PCT Pub. Date Apr. 19, 2018.
Claims priority of provisional application 62/420,650, filed on Nov. 11, 2016.
Claims priority of provisional application 62/411,101, filed on Oct. 21, 2016.
Claims priority of provisional application 62/406,666, filed on Oct. 11, 2016.
Prior Publication US 2023/0203602 A1, Jun. 29, 2023
Int. Cl. C12Q 1/70 (2006.01); G01N 33/569 (2006.01)

CPC C12Q 1/70 (2013.01) [G01N 33/56983 (2013.01); C12Q 2600/158 (2013.01); G01N 2333/7158 (2013.01)]

12 Claims

1. A method for detecting a respiratory virus in a patient, the method comprising:

a. analyzing a viral transport medium sample to determine a level of at least one nasal virus-induced molecule and a level of a housekeeping gene by measuring mRNA;

b. normalizing the level of the at least one nasal virus-induced molecule to the level of the housekeeping gene to determine a normalized level of the at least one nasal virus-induced molecule; and

c. comparing the normalized level of the at least one nasal virus-induced molecule with a predetermined reference level for the at least one nasal virus-induced molecule;

wherein the method further comprises determining the level of interferon lambda 1 (IFNL1) as an indicator of sample quality; and

wherein if the normalized level of the at least one nasal virus-induced molecule is above the respective reference level, the patient is determined to have a respiratory viral infection.