	US 11,965,217 B2
	Method and kit for detecting Mycobacterium tuberculosis
Yi-Chen Li, Taoyuan (TW); Chih-Cheng Tsou, Taoyuan (TW); Min-Hsien Wu, Taoyuan (TW); Hsin-Yao Wang, Taoyuan (TW); and Chien-Ru Lin, Taoyuan (TW)
Assigned to DELTA ELECTRONICS, INC., Taoyuan (TW)
Filed by Delta Electronics, Inc., Taoyuan (TW)
Filed on May 24, 2021, as Appl. No. 17/328,762.
Claims priority of provisional application 63/054,417, filed on Jul. 21, 2020.
Prior Publication US 2022/0025441 A1, Jan. 27, 2022
Int. Cl. C12Q 1/68 (2018.01); C12Q 1/686 (2018.01); C12Q 1/689 (2018.01)

CPC C12Q 1/689 (2013.01) [C12Q 1/686 (2013.01)]

10 Claims

1. A method for detecting Mycobacterium tuberculosis, comprising a step of performing a nested qPCR assay to a specimen, wherein the nested qPCR assay comprises a first round of amplification using external primers and a second round of amplification using internal primers and a probe, the external primers consist of sequences of SEQ ID NOs. 1 and 2, and the internal primers and the probe consist of sequences of SEQ ID NOs. 3 to 5.