US 11,965,209 B2
Method for obtaining structural information concerning an encoded molecule and method for selecting compounds
Thomas Thisted, Frederikssund (DK); Mikkel Dybro Lundorf, Copenhagen (DK); Per-Ola Freskgard, Norrkorping (SE); and Torben Ravn Rasmussen, Ballerup (DK)
Assigned to NUEVOLUTION A/S, Copenhagen (DK)
Filed by NUEVOLUTION A/S, Copenhagen (DK)
Filed on Sep. 9, 2021, as Appl. No. 17/470,923.
Application 17/470,923 is a continuation of application No. 10/572,644, granted, now 11,118,215, previously published as PCT/DK2004/000630, filed on Sep. 17, 2004.
Claims priority of provisional application 60/509,268, filed on Oct. 8, 2003.
Claims priority of provisional application 60/504,748, filed on Sep. 22, 2003.
Claims priority of application No. PA 2003 01356 (DK), filed on Sep. 18, 2003; and application No. PA 2003 01485 (DK), filed on Oct. 8, 2003.
Prior Publication US 2022/0205027 A1, Jun. 30, 2022
Int. Cl. C12Q 1/6837 (2018.01); C12N 15/10 (2006.01); C12Q 1/6811 (2018.01)
CPC C12Q 1/6837 (2013.01) [C12N 15/1068 (2013.01); C12Q 1/6811 (2013.01)] 20 Claims
 
1. A method of identifying a dimer compound that binds to different molecular targets, the method comprising the steps of:
i) providing a first library of bifunctional complexes, each complex comprising a first display molecule and a first identifier oligonucleotide;
ii) exposing the first library to an environment comprising a first target, and identifying first display molecules that bind to the first target with a predetermined binding affinity;
iii) providing a second library of bifunctional complexes, each complex comprising a second display molecule and a second identifier oligonucleotide;
iv) exposing the second library to an environment comprising a second target, and identifying second display molecules that bind to the second target with a predetermined binding affinity;
v) forming dimer compounds by linking first and second display molecules identified in steps ii) and iv), respectively;
vi) exposing the dimer compounds to an environment comprising the first and second targets; and
vii) identifying dimer compounds binding the first and second targets with a predetermined binding affinity.